Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Apr 13;8:432. doi: 10.3389/fimmu.2017.00432

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017 Morgan and Davis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Glucocorticoid treatment suppresses interferon-γ (IFN-γ) production and secretion from natural killer (NK) cells. Primary human NK cells were pretreated with a vehicle control or Dex (100 nM) for 1 h, then either, remained (A) unstimulated, or were stimulated with (B) interleukin-12 (IL-12) (10 ng/ml), (C) interleukin-18 (IL-18) (100 ng/ml), (D) IL-2 (200 U/ml), (E) interleukin-15 (IL-15) (5 ng/ml), (F) IL-12 + IL-15, (G) IL-12 + IL-18, (H) IL-12 + IL-15 + IL-18, or (I) IL-2 + IL-12 for 18 h. IFN-γ release was measured by enzyme-linked immunosorbent assay. Graphs (mean ± SD) combine data for (A) five, (B) five, (C) three, (D,E) four, (F–H) three, or (I) four independent experiments. Production of IFN-γ and tumor necrosis factor-α (TNF-α) mRNA was analyzed by RT-qPCR. Data are normalized to the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase and displayed as a fold change over the unstimulated vehicle control (mean ± SD), for (A) nine, (B–D) three, (E) four, (F–H) three, or (I) eight independent experiments for IFN-γ and (A) four, (B–D) three, (E) four, (F–H) three, or (I) four independent experiments for TNF-α production. Samples are compared by unpaired, two-tailed Student’s t-test (*p < 0.005; **p < 0.005; ***p < 0.0005; ****p < 0.0001; ns, not significant).

Glucocorticoid treatment suppresses interferon-γ (IFN-γ) production and secretion from natural killer (NK) cells. Primary human NK cells were pretreated with a vehicle control or Dex (100 nM) for 1 h, then either, remained (A) unstimulated, or were stimulated with (B) interleukin-12 (IL-12) (10 ng/ml), (C) interleukin-18 (IL-18) (100 ng/ml), (D) IL-2 (200 U/ml), (E) interleukin-15 (IL-15) (5 ng/ml), (F) IL-12 + IL-15, (G) IL-12 + IL-18, (H) IL-12 + IL-15 + IL-18, or (I) IL-2 + IL-12 for 18 h. IFN-γ release was measured by enzyme-linked immunosorbent assay. Graphs (mean ± SD) combine data for (A) five, (B) five, (C) three, (D,E) four, (F–H) three, or (I) four independent experiments. Production of IFN-γ and tumor necrosis factor-α (TNF-α) mRNA was analyzed by RT-qPCR. Data are normalized to the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase and displayed as a fold change over the unstimulated vehicle control (mean ± SD), for (A) nine, (B–D) three, (E) four, (F–H) three, or (I) eight independent experiments for IFN-γ and (A) four, (B–D) three, (E) four, (F–H) three, or (I) four independent experiments for TNF-α production. Samples are compared by unpaired, two-tailed Student’s t-test (*p < 0.005; **p < 0.005; ***p < 0.0005; ****p < 0.0001; ns, not significant).