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. 2017 Apr 13;11:25. doi: 10.3389/fncir.2017.00025

Figure 1.

Figure 1

The distribution of Atoh1 LacZ is shown by ß-galactosidase staining in Atoh1 heterozygous (Atoh1+/−; A,C) and Atoh1−/− littermates (Atoh1−/−; B,D) at embryonic day (E) 10.5 (A,B), E18.5. (C,D) and effects of Pax2-cre mediated deletion of Atoh1 (E,F). Between E10.5 and E18.5 the rhombic-lip shows migratory cells to the isthmus, pons, cerebellum, and cochlear nuclei in Atoh1+/− (A,C) but not in Atoh1−/−(B,D). Consistent with previous detailed analysis (Wang et al., 2005; Rose et al., 2009) there is absence of auditory nuclei in the Atoh1 null mice (C,D) leaving only the Atoh1-LacZ stain along the rhombic lip. Comparison of sections at cochlear nerve entry of control and Pax2-cre, Atoh1 f/f. shows profound reduction likely due to an unclear mix of afferent fiber loss and direct and indirect degeneration of cochlear nucleus neurons (E,F). CB, cerebellum; DCN, Dorsal cochlear nuclei; DCN, dorsal cochlear nucleus; PVCN, postero-ventral cochlear nucleus; VCN, Ventral cochlear nuclei; VIII, VIII nerve root. Bar indicates 100 μm.