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. 2005 Jan;73(1):652–656. doi: 10.1128/IAI.73.1.652-656.2005

FIG. 2.

FIG. 2.

Expression of beta2-toxin or atypical beta2-toxins in concentrated CSF from C. perfringens isolates. One microgram of HIS-atypical beta2-toxin (lane 1) or concentrated CSF from C. perfringens strains (lanes 2 to 9) was subjected to electrophoresis on SDS-10% polyacrylamide gels. CSF from C. perfringens strain 690D (porcine type A) (lane 2), strain 13 (lane 3), JGS4142 (lane 4), JGS1604 (canine type A) (lane 5), JGS1984 (unknown type origin B) (lane 6), JGS1880 (lane 7), JGS4152 (lane 8), and cpb2-negative, porcine type A (negative control) (lane 9) were used. The separated proteins were transferred to nitrocellulose by Western blotting and immunostained with a 1/20 dilution of MAb 9E10B. The positions of molecular mass standards (in kilodaltons) are shown to the left of the gel. The presence of consensus (C) or atypical (A) cpb2 genes or the absence of cpb2 (−) in that strain is indicated below the gel. The positions of HIS-atypical beta2-toxins (arrow) and beta2-toxins (arrowhead) are indicated to the right of the gel.