FIG. 4.

Neutralization with anti-ICAM-1 MAb inhibits the expression of IL-8 mRNA in C. albicans-infected HGECs. HGECs were preincubated with 5, 10, or 20 μg of control IgG or anti-ICAM-1 MAb per ml for 30 min, followed by culture with C. albicans or medium alone for 8 h. Total RNA was isolated from each sample, and levels of IL-8 transcript were determined by real-time RT-PCR analysis. Values given are means ± standard errors of the means (n-fold increases) in the IL-8 gene expression level compared to that in uninfected HGECs for three separate experiments. *, significant difference compared with the value for the group of HGECs infected with C. albicans without MAb treatment (P < 0.01, analysis of variance [Dunnett's multiple-comparison test]).