Table 1.

Antibodies used in the study

Primary antibody	Host	Manufacturer; catalog no.; dilution	Secondary antibody
Enhanced chemiluminescent (ECL) detection
Anti-phospho-S6 (P-S6) Ser235/236	Rabbit	Cell Signaling Technology, Beverly, MA; #4858; 1:500 in 5 % BSA in TBS-Ta	Anti-rabbit IgG, HRP-conjugated; Cell Signaling Technology, Beverly, MA; #7074; 1:10000 in 5 % nonfat milk in TBS-T
Anti-S6 (S6)	Rabbit	Cell Signaling Technology, Beverly, MA; #2217; 1:1000 in 5 % BSA in TBS-T
Anti-mTOR	Rabbit	Cell Signaling Technology, Beverly, MA; #2983; 1:1000 in 5 % BSA in TBS-T
Anti-caspase-3	Rabbit	Cell Signaling Technology, Beverly, MA; #9662; 1:1000 in 5 % BSA in TBS-T
Anti-GFP	Rabbit	MBL International Corporation, Geel, Belgium; #598; 1:2000 in 5 % nonfat dry milk in TBS-T
Anti-Elmo1	Rabbit	Eurogentec, Liege, Belgium; custom orderb; 1:500 in 5 % nonfat dry milk in TBS-T
Anti-α-tubulin	Mouse	Sigma-Aldrich, St. Louis, MO; #T5168; 1:20,000 in 5 % nonfat dry milk in TBS-T	Anti-mouse IgG, HRP-conjugated; Cell Signaling Technology, Beverly, MA; #7076; 1:10000 in 5 % nonfat milk in TBS-T
Anti-β-actin-HRP	Mouse	Sigma-Aldrich, St. Louis, MO; A3854; 1:15,000 in 5 % nonfat dry milk in TBS-T	N/A
Immunofluorescence
Anti-phospho-S6 (P-S6) Ser235/236	Rabbit	Cell Signaling Technology, Beverly, MA; #4858; 1:200 in 1 % donkey serum in PBS-Tc	Anti-rabbit IgG Alexa Fluor 488; Life Technologies, Carlsbad, CA; A-21206; 1:500 in 1 % donkey serum in PBS-T
Anti-c-Fos	Rabbit	Santa Cruz Biotechnology, Santa Cruz, CA; #7202; 1:200 in 1 % donkey serum in PBS-T
Anti-GFAP	Mouse	Millipore, Darmstadt, Germany; #04-1031; 1:500 in 1 % donkey serum in PBS-T
Anti-GFP	Mouse	MBL International Corporation, Geel, Belgium; #598; 1:1000 in GDB bufferd

^aTris-buffered saline (TBS-T) (50 mM Tris, 150 mM NaCl), pH 7.6 + 0.1 % Tween 20

^bElmo1 peptide sequence taken for rabbit immunization: aa 109–124: GDLEESPQGEVPHDSL

^cPhosphate-buffered saline (PBS-T) (137 mM NaCl, 2.7 mM KCl, 10 mM Na₂HPO₄, 2 mM KH₂PO₄), pH 7.4 + 0.2 % Triton X-100

^dGDB buffer (0.2 % gelatin, 0.8 M NaCl, 0.5 % Triton X-100, 30 mM phosphate buffer), pH 7.4)