Table 1.
Primer sets.
| Gene | Primer Sets | Tm (°C) | Product length |
|---|---|---|---|
| Adra2 | |||
| F | CAGCTCGCTGAACCCTGTTA | 59.96 | 117 |
| R | CACGATGCGTTTTCTGTCCC | 60.04 | |
| AT1A | |||
| F | AGTTGGGAGGGACTGGATGA | 59.88 | 149 |
| R | GTTAAGTCCGGGAGAGCAGC | 60.46 | |
| AT1B | |||
| F | GCAGGGAGTAACAGAGACCA | 58.73 | 134 |
| R | GTGAATTCAAAATGCACCCGT | 57.97 | |
| AT2 | |||
| F | TTTTAAGGAGTGCATGCGGGA | 60.27 | 148 |
| R | GGTAATGTTTCTGCTGGTGGC | 59.8 | |
| EEF2 | |||
| F | ACATGTCAGTCATCGCCCAT | 59.46 | 166 |
| R | GAGATGGCGGTGGATTTGATTG | 59.97 | |
| IL-6 | |||
| F | CGGCCTTCCCTACTTCACAA | 59.68 | 149 |
| R | GCCATTGCACAACTCTTTTCTCA | 60.24 | |
| iNOS | |||
| F | CCTGCTTTGTGCGAAGTGTC | 60.04 | 140 |
| R | CCCTTTGTGCTGGGAGTCAT | 59.96 | |
| MCP-1 | |||
| F | CTTCTGGGCCTGCTGTTCA | 59.93 | 127 |
| R | CCAGCCTACTCATTGGGATCA | 59.23 | |
| MMP-2 | |||
| F | AACGGTCGGGAATACAGCAG | 60.11 | 125 |
| R | GTAAACAAGGCTTCATGGGGG | 59.18 | |
| MMP-9 | |||
| F | CAGCCGACTTTTGTGGTCTTC | 59.74 | 87 |
| R | ATAGCGGTACAAGTATGCCTCTG | 59.99 | |
| NF-κB | |||
| F | GGCAGTGACGCGACGA | 59.73 | 129 |
| R | AAACAGATCGTCCATGGTCAGG | 60.36 | |
| TNF-α | |||
| F | TAGCCCACGTCGTAGCAAAC | 60.39 | 136 |
| R | ACAAGGTACAACCCATCGGC | 60.32 | |
Adra2, α2 adrenergic receptors; AT1A, type 1A angiotensin receptor; AT1B, type 1B angiotensin receptor; AT2, type 2 angiotensin receptor; EEF2, eukaryotic translation elongation factor 2; F, forward; IL-6, Interleukin-6; iNOS, inducible nitric oxide synthase; MCP-1, monocyte chemoattractant protein-1; MMP, matrix metalloproteinase; NF-κB, Nuclear factor-kappa B; R, reverse; Tm, melting temperature; TNF-α, tumor necrosis factor-alpha.