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. 2017 Apr 13;8:511. doi: 10.3389/fpls.2017.00511

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Copyright © 2017 Li, Chen, Shi, Wang, Fu, Qiu and Cui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Soluble protein profiles show the effect of the reductant ME on protein extraction in Phragmites ecotypes. The soluble proteins were extracted in the absence (A) or presence (B) of ME. In order to alleviate the saturation effect of highly abundant leaf proteins, only 200-μg protein samples were separated based on the IPG strips (24 cm, pH 4-7) and 12.5% SDS-PAGE gel. The gels were stained with CBB R-250. Rubisco contents were calculated according to the intensity levels of the Rubisco protein spots, including the large and small subunits, which are circled on the maps.