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. 2017 Apr 13;8:567. doi: 10.3389/fpls.2017.00567

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Copyright © 2017 Gupta, Nigam, Dahuja, Kumar, Vinutha, Sachdev and Praveen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relative expression level of newly identified target genes (UDP-glucosyltransferase: Glyma.08G181000; UDP-glucosyltransferase: Glyma.10G224000; 4-coumarate-CoA ligase: Glyma.10G197900; isoflavone 7-O-glucosyltransferase: Glyma.09G127200) along with their corresponding miRNAs (Gma-miR12, Gma-miR24, Gma-miR26, and Gma-miR28) in NRC7 soybean cultivar at four developmental stages, i.e., (A) 35 DAF; (B) 45 DAF; (C) 55 DAF, and (D) 65 DAF. Control refers to Glyma.02G279600 and Gma-miR29 for target and miRNA, respectively, which was used to calculate ΔΔCt value throughout all the stages. Three reference genes (EF 1α 2a, Cyclophillin and Actin 2/7) were used to calculate accurate normalized value using Genorm software. The expression level of miRNAs as well as its target gene was normalized to the normalized value of reference genes on a logarithmic scale. Error bar represents ± SE of three independent biological replications.