Table 1.
Newly identified five miRNAs of isoflavone biosynthetic pathway from Soybean ESTs.
| Sr. No. | miRNA header | Mature miRNA | Mature length | Precursor length | GC% | MFE (kcal/mol) | MFEI | AMFEI | AU% | Homologs miRNAs |
|---|---|---|---|---|---|---|---|---|---|---|
| (1) | >Gma_miRNA12 | AGACAGUUAUUUUGGGACGGA | 21 | 121 | 41.32231 | -44.0 | -0.88 | -36.36 | 58.67769 | hvu-miR5049c |
| (2) | > Gma _miRNA24 | UCUUGAAGUCUCGCUUGCAG | 20 | 120 | 49.16667 | -36.3 | -0.61 | -30.25 | 50.83333 | bdi-miR7741-5p.2 |
| (3) | > Gma _miRNA26 | UAAUUGUCGCAGUUUUGAACU | 21 | 121 | 31.40496 | -36.1 | -0.95 | -29.83 | 68.59504 | bdi-miR5180b |
| (4) | > Gma _miRNA28 | UCUGUACCAUAAUAUAAGAC | 20 | 120 | 40 | -35.1 | -0.73 | -29.25 | 60 | huv-miR1130 |
| (5) | > Gma _miRNA29 | UAGAUACAUCCAUAUGUAGA | 20 | 120 | 35 | -34.4 | -0.81 | -35.66 | 65 | tae-miR1122a |