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1H-15N BEST-TROSY correlation spectra of the different Ascl1 fragments designed and used for this study. (A) Residues 1–78. (B) Residues 1–117. (C) Residues 114–183 (the bHLH domain). (D) Residues 118–236. The spectra shown were recorded at an optimized sample temperature at which the NMR assignment was performed. Correlation peaks in the different NMR spectra are annotated by the corresponding residue number and type. To see this figure in color, go online.
