Figure 5.

(A) ¹H-¹⁵N TROSY spectrum of pPdc in the absence (red) and presence (black) of 14-3-3ζΔC. For better clarity, overlapping signals in the center part of the spectrum are not labeled. The TROSY spectrum corresponding to the region of phosphoserine resonances is depicted in detail in the inset. The pPdc:14-3-3ζΔC molar ratio was 1:2. The overall numbers of 164 and 136 signals were assigned in the case of pPdc and the pPdc:14-3-3ζΔC complex, respectively. (B) Combined chemical-shift difference calculated from the chemical shifts observed in the ¹H-¹⁵N TROSY spectra of pPdc and pPdc:14-3-3ζΔC. Combined chemical-shift differences (Δδ_comb) were calculated as $\Delta {\delta }_{comb}=\sqrt{\Delta {\delta }_{1H}^2+\left(\Delta {\delta }_{15N}^2/36\right)}$. The compact stretch of five or more residues that were present in the pPdc spectrum, but were not observed in the ¹H-¹⁵N TROSY spectrum of the pPdc:14-3-3ζΔC complex, is highlighted in red, and the isolated residues missing in the spectrum are depicted in gray. To see this figure in color, go online.