Figure 6.

PREs of ¹⁵N pPdc in complex with 14-3-3ζΔC single C189. The IPSL spin label was attached to C189 of the 14-3-3ζΔC protein and a ¹H-¹⁵N TROSY spectrum was acquired with IPSL in an oxidized or reduced state. The bars indicate the ratios of peak intensities for individual amino acids in the pPdc sequence in the spectra acquired on samples with the label in the oxidized or reduced form, respectively. The gray background shows the amino acid residues for which data are not available from either spectrum. A zoomed view of the region corresponding to the Pdc sequence 15−40 is shown in the upper panel. The intensity ratios do not converge to one even in the case of residues that are not affected by the presence of the IPSL label, due to the fact that a lower concentration of pPdc:14-3-3ζΔC complex was used in the sample with the IPSL label in the oxidized state as compared with the sample with IPSL in the reduced state. To see this figure in color, go online.