Table 1.
Structural Parameters Determined from the SAXS Data
| c (mg.mL−1) | ca (μM) | Rg (Å)b | Rg (Å)c | Dmax (Å) | Vp (nm3)d | MMMoW (kDa)e,f | |
|---|---|---|---|---|---|---|---|
| Complex | 16.8 | 193 | 32.1 ± 0.5 | 32.9 ± 0.1 | 109 | 108 | 77.4 |
| 10.9 | 125 | 33.6 ± 0.1 | 34.1 ± 0.1 | 120 | 114 | 81.3 | |
| 6.3 | 72 | 34.3 ± 0.8 | 35.2 ± 0.1 | 125 | 118 | 82.4 | |
| 5.6 | 64 | 34.4 ± 0.8 | 35.1 ± 0.1 | 125 | 117 | 82.2 | |
| 14-3-3ζΔC | 11.1 | 196 | 28.2 ± 0.1 | 28.3 ± 0.1 | 84 | 85 | 56.6 |
| 8.4 | 148 | 28.5 ± 0.1 | 28.5 ± 0.1 | 85 | 87 | 57.8 | |
| 4.3 | 76 | 28.8 ± 0.1 | 28.8 ± 0.1 | 86 | 89 | 59.6 |
a
Molar concentration of the 14-3-3ζΔC dimer and the pPdc:14-3-3ζΔC complex with 1:2 molar stoichiometry.
b
Calculated using the Guinier approximation (25).
c
Calculated using the program GNOM (26).
d
Excluded volume of the hydrated particle (the Porod volume).
e
Molecular mass calculated from the experimental scattering data using the program SAXSMoW (41).
f
The theoretical molecular masses of the 14-3-3ζΔC dimer and the pPdc:14-3-3ζΔC complex (with 1:2 stoichiometry) are 56.6 and 86.9 kDa, respectively.