Figure 3.

Binding free energy parameter grids used to reduce parameter search space and identify χ² minima. (A) Grid of coupling free energy (ΔG_γ) versus the free energy of binding to a reference state with no occupied neighbors (ΔG₀), for the six-parameter additive model. (Blue cross) Global minimum. (B) Coupling free energies for the nine-parameter model of nonadditive cooperativity, of sites flanked by one (ΔG_oe) and two (ΔG_oo) occupied neighboring sites, versus the reference state ΔG₀. (Red crosses) Values of fits to 50 random bootstrap datasets were used to estimate parameter uncertainties, using the grid minimum as a starting point. To see this figure in color, go online.