Figure 5.

Populations of Trp+TRAP species during the ITC titration predicted by the nonadditive model using the best-fit parameters from Table 1. (A) The relative abundance at each titration point, of TRAP₁₁ species with 0–11 bound Trp, colored purple to red for the ITC titration experiment performed at 40°C (starting TRAP₁₁ concentration = 6.81 μM). (B) The relative abundance of empty sites (black), occupied sites with no bound neighbors (red), occupied sites with one bound neighbor (green), and occupied sites with two bound neighbors (blue), as a function of Trp/TRAP ratio during ITC titrations over a range of temperatures. To see this figure in color, go online.