Figure 2. Unrooted phylogenetic tree of all non-redudant cyt-homologous toxins that were retrieved from Genbank, Uniprot and specific genome databases.

Phylogenetic reconstruction, on aligned protein sequences followed a PhyML method⁵⁰ with a WAG 4-rate class model. The early three separate clades are labeled evf, volvatoxin and (other) bacterial cyt clades. Labels are built to allow both easy reading and non-ambiguous peptide identification: Bacillus thuringiensis toxins are not preceded by the species suffix (otherwise: three letter suffix-id, Gsp for Genus species), follow the Crickmore holotype classification, and labels include the Uniprot accession number for non-Bt species.