Figure 2. Analysis by SPR of protein-protein interaction.

(A) Real time association-dissociation sensorgrams of the macrophage extract with immobilized PtpA D126A. Values are expressed as percentage with 100% corresponding to 90 RU. Inset: association-dissociation sensorgrams corresponding to the interaction of macrophage extract with immobilized PtpA wt. (B) Effect of Na₃VO₄ on the association of macrophage components to PtpA D126A. Results are shown as the maximal association response units (RU, expressed as the percentage relative to the response measured without inhibitor) achieved at increasing concentrations of the inhibitor Na₃VO₄. Accordingly, a 100% response corresponds to the maximal RU obtained by injection of the macrophage extract without inhibitor. Inset: association-dissociation sensorgrams of macrophage extract mixed with 25 mM sodium Na₃VO₄. These sensorgrams are representative of the results obtained in more than three analytical replicates and two biological replicates performed with different batches of purified PtpA and macrophage extracts.