Table 3. Kinetic parameters for acetyl-CoA binding for wild-type SsArd1 and its mutants.

Enzyme	Km, AcCoA	kcat, AcCoA	kcat/Km
	μM	min−1	min−1 μM−1
Wild type	67.17 ± 7.48	33.57 ± 1.65	5.02×10−1 ± 0.31
R100A	466.98 ± 50.83	28.70 ± 2.69	6.15×10−2 ± 0.09
T105A	195.89 ± 12.27	34.91 ± 1.23	1.78×10−1 ± 0.04
R132A	279.37 ± 75.86	33.36 ± 3.88	1.23×10−1 ± 0.21