Figure 2.

Gata3 Is Required for Branching Morphogenesis and Prostate Epithelial Homeostasis

(A) Ductal architecture of control and Nkx3-1^Cre/+;Gata3^flox/flox prostates and individual lobes at 2 weeks of age as shown by β-galactosidase staining. Scale bars, 1 mm.

(B) Quantification of the number of prostate ducts and branch points in control and Nkx3-1^Cre/+;Gata3^flox/flox prostates.

(C) H&E staining of developing (2-week-old) prostate sections in control and Nkx3-1^Cre/+;Gata3^flox/flox mice. Scale bars, 20 μm.

(D) H&E staining of developing (6-week-old) prostate sections in control and Nkx3-1^Cre/+;Gata3^flox/flox mice. Black bars indicate the thickness of the epithelial layer. Scale bars, 20 μm.

(E) Quantification of proliferating (phospho-histone H3-positive cells) and apoptotic (TUNEL staining) cells in control and Nkx3-1^Cre/+;Gata3^flox/flox tissue at P14. ^∗p < 0.05.

Representative images and quantifications are from three control and Nkx3-1^Cre/+;Gata3^flox/flox prostates, except in (B), where error bars represent SE from two control and Nkx3-1^Cre/+;Gata3^flox/flox prostates.