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. 2017 Mar 9;8(4):1018–1031. doi: 10.1016/j.stemcr.2017.02.004

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Loss of Gata3 Disrupts Prostate Epithelial Polarity and Increases the Double-Positive Progenitor Cell Population

(A) Immunofluorescence staining of basolateral (B-catenin and E-cadherin) and apical (ZO-1) markers in control and Nkx3-1^Cre/+;Gata3^flox/flox prostate tissue.

(B) Immunofluorescence staining of basal (CK5) and luminal (CK8/18) cell markers in 2-week-old control and Nkx3-1^Cre/+;Gata3^flox/flox prostate and quantification of the number of single-positive basal (CK5⁺), luminal (CK8/18⁺), and double-positive intermediate progenitor (CK5⁺; CK8/18⁺) cells per millimeter of ductal circumference.

(C) Immunofluorescence staining of basal (CK5) and luminal (CK8/18) cell markers in 6-week-old control and Nkx3-1^Cre/+;Gata3^flox/flox prostate and quantification of the number of single-positive basal (CK5⁺), luminal (CK8/18⁺), and double-positive intermediate progenitor (CK5⁺; CK8/18⁺) cells per millimeter of ductal circumference. Arrows in (B and C) indicate double-positive cells.

^∗p < 0.05, ^∗∗p < 0.01. Representative images and quantifications are from four control and Nkx3-1^Cre/+;Gata3^flox/flox prostates. Error bars represent SE from three (B) and four (C) control and Nkx3-1^Cre/+;Gata3^flox/flox prostates. Scale bars, 10 μm.