Figure 4.

Loss of Gata3 Randomizes Spindle Orientation in Basal Progenitor Cells without Affecting Their Proliferation Rate

(A) Percentage of dividing cells in each epithelial lineage in control and Nkx3-1^Cre/+;Gata3^flox/flox prostate tissue.

(B) Analysis of the phases of mitosis in control and Nkx3-1^Cre/+;Gata3^flox/flox prostate tissue.

(C) Mitotic spindle orientation was measured in basal (CK5⁺), luminal (CK8/18⁺), and double-positive cells (CK5⁺; CK8/18⁺) of control and Nkx3-1^Cre/+;Gata3^flox/flox (red box) prostate tissue by γ-tubulin (spindle poles) and phospho-histone H3 (dividing cells) immunofluorescence staining.

(D) Quantification of spindle orientations in the three cell types in control and Nkx3-1^Cre/+;Gata3^flox/flox prostate.

Dashed lines indicate axis of cytokinesis. ^∗p < 0.05, ^∗∗∗p < 0.001; ns, not significant. Quantifications are from four control and Nkx3-1^Cre/+;Gata3^flox/flox prostates. Error bars in (A) represent SE from four control and Nkx3-1^Cre/+;Gata3^flox/flox prostate replicates. n values in (D) represent the total number of cell divisions quantified per cell type. Scale bars, 5 μm.