Figure 2.

Higher Resistance of ZL55-SO^high EGFP(+) Cells Toward cis-Pt and 5-FU and a Lower One Toward VS-6063

(A and B) Increased viability of ZL55-SO-P2 versus ZL55-SO cells exposed to cis-Pt (left panel) (A). From the curves derived from sorted ZL55-SO^low and ZL55-SO^high cells (right three panels), IC₅₀ values for cis-Pt, 5-FU, and VS-6063 were calculated from three independent experiments and are shown in (B). ^∗∗∗p < 0.001; one-way ANOVA; n.s., not significant. ZL55-SO^high cells display higher susceptibility to VS-6063.

(C) Real-time growth curves show the strong effect of cis-Pt (1.25 μM) on ZL55-SO cell proliferation/survival, while ZL55-SO-P2 cells are almost completely resistant. One representative experiment from three independent experiments.

(D) Bright field and fluorescence images showing the effect of cis-Pt on ZL55-SO cells; the fraction of EGFP(+) cells is increased after cis-Pt exposure (right panels).