Figure 4.

Increased Tumor Growth of Human ZL55-SO^high EGFP(+) Cells in NOD/SCID Gamma Mice

(A) ZL55-SO^low and ZL55-SO^high cells (100,000/mouse) were injected i.p. into NOD/SCID gamma mice. Mice injected with ZL55-SO^high cells show an increased number/density of tumor nodules, especially visible on the visceral pleura (right images).

(B) The percentage of tumor nodules covering the surface of the visible parietal and visceral peritoneum is 9.1% for ZL55-SO^low cells and 27.8% for ZL55-SO^high cells (^∗∗∗p < 0.001; one-way ANOVA). Average of four to five animals per group; error bars represent SD.

(C) Tumor masses were histologically analyzed; a tumor nodule on the liver surface shows highly invasive edges (arrow). See also Figure S3.