Figure 1.

Microarray Analysis of the Effect of Pro-inflammatory and Anti-inflammatory Macrophages on Mesenchymal Stromal Cells

MSCs were cultivated alone (MSC), or with pro-inflammatory (M1MФ; M1-MSC) or anti-inflammatory (M2MФ; M2-MSC) MSCs for 24 hr. After magnetic separation, microarray assay was realized.

(A) Principal component analysis of total gene expression. Draw of ellipsoids encompassing the individual data points: MSCs (green; 3 samples), M1-MSCs (red; 3 samples), M2-MSCs (blue; 4 samples). Note that the dots for MSCs (green) and M2-MSCs (blue) are grouped together (Partek).

(B) Venn diagram of total genes modulated in MSCs after contact with M1MФ (M1-MSCs) or M2MФ (M2-MSCs). Gene expression analysis (genes with a fold change ≥2 and false discovery rate [ANOVA] <0.05).

(C) Functional analysis of 559 modulated genes in M1-MSCs.

(D) Heatmap based on the expression of genes coding for proteins involved in immunosuppressive capacities of MSCs (see log₂ expression scale).

See also Figure S3.