Figure 5.

M1MФ Interacted with MSCs but Did Not Induce Cytoskeletal Polarization

(A) MSCs were cultivated with M1MФ for 15 min and cells were fixed and labeled with anti-tubulin antibody (green). Secondary antibodies were used as a negative control.

(B) M1MФ-MSC conjugates stained with antibodies for actin (green) and tubulin (blue) (top). M1MФ-MSC conjugate stained with antibodies for tubulin (green) and CD54 (blue) (bottom). Images from bright-field (DIC) and overlapped staining (Merge) are shown. MФ = M1MΦ.

(C) M2MФ-MSC conjugates stained with antibodies for actin (green) and tubulin (blue). Images are representative of three independent experiments.