Figure 6.

M1MФ Interacting with MSCs Increased Intracellular Ca²⁺ Mobilization in MSCs

MSCs were cultivated with or without M1MФ or M2MФ for 24 hr. Cells were labeled with Fura-2-AM, and 340-/380-nm fluorescence signal ratio was recorded. All snapshots represent the 340/380 ratio intensity by a pseudocolor scale. In the first image of each snapshot, MSCs are indicated with white dots and macrophages are highlighted by an orange line. The calcium flux is indicated with the arrow.

(A–C) Snapshot sequence of Movies S1 (A), S2 (B), and S3 (C).

(D) The bars represent the mean minimum and maximal intensity of 340/380 ratio ± SEM (n = 5 independent experiments). ^∗∗p < 0.01, ^∗∗∗p < 0.001.