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. 2017 Mar 16;8(4):1046–1061. doi: 10.1016/j.stemcr.2017.02.012

Figure 1.

Figure 1

Split-Cre Virus-Mediated Dicer Ablation In Vivo Impairs Neuronal Differentiation and Survival but Not Astrogliogenesis

(A) Schematic representation of the experiment.

(B) qRT-PCR quantification of Dicer mRNA from FACS-sorted Td-Tomato+ aNSCs 2 months after split-Cre virus injection.

(C and E) Representative micrographs showing recombined Td-Tomato/BrdU double-positive cells from Dicer WT and cKO mice 1 month after BrdU injection (C), co-expressing NeuN (E, left panel), GFAP (E, middle panel) and S100b (E, right panel). Yellow arrowheads show Td-Tomato/BrdU double-positive cells co-expressing NeuN, GFAP, or S100b.

(D) Percentage of Td-Tomato+ cells expressing BrdU after 10 days, or 1 month after BrdU injections.

(F–H) Percentage of Td-Tomato/BrdU double-positive cells co-expressing NeuN (F), DCX (G), or S100b (H) 10 days or 1 month after BrdU injections.

ML, molecular layer; GCL, granular cell layer; SGZ, subgranular zone; H, Hilus. Data are expressed as mean ± SEM, n = 4–6 mice per group. Unpaired t test was used for Dicer mRNA expression analysis. One-way ANOVA Bonferroni as post hoc was used to analyze cell marker quantification. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Scale bars, 20 μm.