Figure 2.

Dicer and miRNAs Are Depleted after Recombination of Dicer^flox Allele in Hippocampal aNSCs In Vitro

(A) Representative micrographs showing Td-Tomato⁺ aNSCs from Dicer WT, Dicer HT, and Dicer cKO mice after nucleofection with Cre recombinase.

(B) PCR Genotyping of Cre-recombined aNSCs, showing the three Dicer genotypes.

(C) qRT-PCR quantification of Dicer mRNA in Cre-recombined aNSCs.

(D) Average of all miRNAs quantified from recombined aNSCs.

Data are expressed as mean ± SEM, n = 3 independent experiments containing three replicates. One-way ANOVA Bonferroni as post hoc: ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Scale bar, 50 μm.