Figure 3.

Loss of Dicer-Dependent miRNA in aNSCs Impairs Neurogenesis and Neuronal Maturation In Vitro

(A) Schematic representation of the protocol and experiment.

(B) Representative micrographs showing recombined Td-Tomato⁺ aNSCs from Dicer WT, Dicer HT, and Dicer cKO mice after 6 DIV with growth factors titration expressing DCX.

(C) Percentage of Td-Tomato⁺ cells expressing DCX.

(D) Representative micrographs showing dendritic morphology of immature newly formed neurons expressing DCX.

Data are expressed as mean ± SEM, n = 3 independent experiments containing three replicates. One-way ANOVA Bonferroni as post hoc: ^∗p < 0.05, ^∗∗∗p < 0.001. Scale bars, 50 μm.