Figure 4.

Loss of Dicer-Dependent miRNAs Does Not Affect Astrogliogenesis of aNSCs In Vitro

(A) Schematic representation of the protocol and experiment.

(B) Representative micrographs showing recombined Td-Tomato⁺ aNSCs from Dicer WT, Dicer HT, and Dicer cKO mice after 6 DIV with 10% fetal bovine serum expressing S100b (upper panels) and GFAP (bottom panels).

(C) Percentage of Td-Tomato⁺ cells expressing astrocyte markers (GFAP and S100b).

(D–F) Relative Nestin (D), GFAP (E), and S100b (F) mRNA quantification with qRT-PCR.

(G–I) Protein quantification of NESTIN (H) and GFAP (I).

Data are expressed as mean ± SEM, n = 3 independent experiments containing three replicates. One-way ANOVA Bonferroni as post hoc: ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001. Scale bar, 50 μm.