Figure 5.

Profiling of miRNA Expression during Neuronal Differentiation of aNSCs In Vitro

(A) Schematic representation of the neuronal differentiation protocol with inducible retrovirus expressing ASCL1 (Ascl1-ERT2-IRES-GFP) and the experiment. Cells were collected during proliferation (P) or differentiation after 7 (D7), 14 (D14), and 21 (D21) DIV.

(B) Heatmap representing the set of miRNAs dynamically regulated upon neuronal differentiation at 7, 14, and 21 DIV. Red indicates high expression and green, low expression.

(C) Fold change of selected miRNAs during differentiation over proliferating cells.

(D) Fold change expression of miRNAs in vivo in FACS-sorted Td-Tomato⁺ cells from ten adult Dicer WT and ten Dicer cKO mice 2 months after split-Cre recombinase virus injection into the DG. n = 3 independent experiments containing three replicates.

Data are expressed as mean ± SEM. Paired t test: ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.