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. 2017 Mar 16;8(4):1046–1061. doi: 10.1016/j.stemcr.2017.02.012

Figure 6.

Figure 6

A Pool of 11 miRNAs Synergistically Rescues Dicer cKO Impairment of Adult Neurogenesis, at the Expense of Astrogliogenesis, In Vitro

(A) Representative micrographs showing aNSCs from Dicer WT and Dicer cKO mice transfected with 250 nM scrambled RNA or total pool (25 nM of each miRNA) after 6 DIV of growth factors withdrawal expressing DCX (upper panel), MAP2 (middle panel), and S100b (bottom panel). Scale bar, 50 μm.

(B) Percentage of DCX-, MAP2-, and S100b-positive aNSCs with respect to DAPI in WT and KO aNSCs transfected with scrambled RNA or total pool.

(C) Proportion of KO aNSCs expressing DCX upon transfection of 250 nM subpool 1 (mir-124-3p + mir-135a-5p), subpool 2 (mir-139-5p + mir-218-5p + mir-411-5p + mir-134-5p + mir-370-3p + mir-382-5p + mir-708-5p), subpool 3 (mir-127-3p + miR-376b-3p), or each miRNA alone with respect to KO control.

(D) Percentage of DCX-positive aNSCs with respect to DAPI after 6 DIV from WT or KO mice transfected with scrambled RNA, total pool, or a pool with ten miRNAs by the withdrawal of individual miRNAs.

(E) mRNA quantification with qRT-PCR from recombined KO aNSCs after 6 DIV.

Data are expressed as mean ± SEM, n ≥ 3 independent experiments containing three replicates. One-way ANOVA with Bonferroni as post hoc test: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.