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. 2017 Mar 16;8(4):1062–1075. doi: 10.1016/j.stemcr.2017.02.013

Figure 6.

Figure 6

DIDO1 Interacts with WWP2 in PE Formation

(A) Left: WT d4EBs stain positive with anti-WWP2 (red) at apical membrane of OCT4 (green)-negative PE cells. Right: overlapping staining of DIDO1 (cyan) with WWP2 (red) on PE cells of WT d4EBs. Scale bar, 50 μm.

(B) WWP2 co-immunoprecipitated with HA-DIDO1 or HA-DIDONT, but not with HA-DIDO3CT.

(C) Co-staining of d4EBs with OCT4 (green) and PE markers FOXA2 (red) and DIDO1 (cyan) in WT d4EBs infected with shRNA-Co (top row) or two clones of shRNA-Wwp2 A and B (center and bottom rows, respectively). Clone A completely lacked PE formation and clone B showed aberrant co-expression of OCT4 and FOXA2 in PE cells (zoomed images 1 and 2). Nuclei (DAPI, blue). Scale bars for EBs, 50 μm.