FIG. 4.

In vivo fluorescence time-lapse analysis of Hhf1p-GFP in C. albicans dyn1. Representative frames of a movie with strain GC17. Cells were pregrown to exponential phase and were mounted on microscopy slides using the same conditions as those used for the time-lapse movie with strain GC12 (Fig. 3). Note the completion of mitosis in a mother cell and postmitotic nuclear migration between 41 and 44 min, as marked by the arrowheads. Time is in hours:minutes. Bar, 10 μm. The movie is available at http://pinguin.biologie.uni-jena.de/phytopathologie/pathogenepilze/index.html.