FIG. 8.

Analysis of nuclear positioning in wild-type and dyn1 mutant strains. Cells were incubated for 10 h under hypha-inducing conditions (CSM in the presence of 0.5 g of proline/liter). DIC images and images showing the DAPI fluorescence of stained nuclei were merged into an overlay. Characteristic nuclear positioning in the wild type (A) shows elongated hyphae with regular nuclear spacing, septal intervals, and one nucleus in the apical compartment, whereas inducing conditions resulted in germ tube formation in the dyn1 mutant (B) in which nuclei were either trapped in the germ cell or did not migrate into the hyphal tip. (C) Hyphal induction using C. albicans strain CAT23 (MAL2p-DYN1:HIS1/dyn1::URA3) in maltose-containing medium shows wild-type-like nuclear positioning, whereas hyphal induction on CSM containing glucose (D) reveals the dyn1 mutant phenotype.