Figure 3.

Genes Annotated as Transcription Factor Activity Are Immediately Enriched Upon Differentiation

(A) Percentage of probes annotated as TF activity during differentiation into osteoblasts and adipocytes of the total differential expressed probes.

(B) Venn diagram of all probes annotated as TF activity (GO: 0003700) that are differentially expressed within the first 3 hr during adipocyte and osteoblast differentiation.

(C) Hierarchical clustering of 133 probes of the gene expression study and annotated as TF activity (depicted in B). Red, upregulated; green, downregulated relative to undifferentiated cells; yellow, significant differentially expressed within 3 hr of osteoblast differentiation; blue, significant differentially expressed within 3 hr of adipocytes differentiation.

(D) GeneMania network analyses of the 77 (88 probes) TFs that were up- or downregulated during osteoblast differentiation. Gray and yellow edges illustrate consolidated pathways and protein domains with edges shared, respectively. Blue nodes are pathways; known osteoblast TFs are marked in red; and green nodes depict the TFs that are specifically regulated during osteogenesis. Arrow see text.

(E) GeneMania network analyses of the 95 (104 probes) TFs that were up- or downregulated during adipocyte differentiation. Edges are colored as in (D). Green nodes are TFs that are specifically regulated during adipogenesis.