Figure 5.

Differentiation of TSC pNSCs into Neurons

(A–G) Immunofluorescence images (A) of neurons differentiated from the patient with TSC and the unaffected controls showing the expression of β-III-TUBULIN and the morphology of the neurons differentiated from the patient with TSC and the unaffected controls as determined using NeurphologyJ (B–G; n = 3 independent experiments, mean ± SEM; ^∗∗∗p < 0.005, ^∗∗∗∗p < 0.001; Student's t test). Scale bar, 20 μm.

(H) Increased S6 phosphorylation in TSC neurons. Scale bar, 100 μm.

See also Figure S5.