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Deletion of ppoC induces sexual development in liquid cultures. Cultures of A. nidulans wild-type (A), ΔppoC (B), and ΔppoA ΔppoC (C) were grown at 37°C in liquid GMM. Abundant presence of Hülle cells after 2 days growth in liquid cultures of ΔppoC and ΔppoA ΔppoC strains (B and C). The double mutant was able to produce mature and fertile cleistothecia after 2 days of growth (C2 and C3). Hu, Hülle cells; Cl, cleistothecium; As, ascospores.
