FIG. 7.

ppoC and ppoA are differentially regulated under asexually (A) and sexually (B) induced cultures. Mycelia of the wild-type strain were synchronized by 18 h of vegetative growth in liquid shaken GMM (time zero) and developmentally induced on solid GMM to obtain asexual (A) and sexual (B) tissue types for RNA isolation at appropriate time intervals under dark or light conditions. Time points represents hours after asexual or sexual induction, respectively. Induction of asexual sporulation was performed under normal aeration conditions. The time point “−2” corresponds to 16 h of vegetative growth culture, 2 h before the transfer to solid medium for asexual induction. For the induction of sexual sporulation 18-h-liquid-grown mycelia were transferred onto solid medium, and the plates were sealed with parafilm for 20 h. After 20 h, the plates were unsealed, and at different time points samples were collected for RNA analysis (time represents hours after induction: 0 to 36 h). The time point “T” corresponds to the sample that was collected at the time of transfer to the solid media and before the initiation of sexual induction. (C) Differential regulation of ppoA and ppoC expression as was demonstrated by their transcript analysis in OE::ppoA (C1) and ΔodeA (C2) strains. Equal loading of total RNA (20 μg) is depicted by ethidium bromide staining of the rRNA. The time points of mycelium harvest are indicated above the lanes.