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Antagonistic transcriptional regulation of fatty acid biosynthetic genes by PpoA and PpoC. Cultures of A. nidulans wild-type, ΔppoA, ΔppoC, and ΔppoA ΔppoC strains were grown at 37°C in stationary liquid GMM for 72 h and analyzed for the expression profile of fatty acid anabolic genes. The biosynthetic role of each gene is depicted in the flow chart of fatty acid biosynthesis in A. nidulans. Equal loading of total RNA (20 μg) is depicted by ethidium bromide staining of the rRNA.
