TABLE 1.
A. nidulans strains used in this study
| Fungal straina | Genotype | Source or reference |
|---|---|---|
| RDIT12.3 | biA1 argB2 metG1 ΔppoA::metG veA | 55 |
| RDIT44.10 | pabaA1 biA1 pyroA4 metG1 veA1 trpc801 | 55 |
| TTMK1.97 | argB2 metG1 ΔppoC::trpC veA1 trpC801 | This study |
| RDIT30.92 | argB2 metG1 veA1 trpC801 | 55 |
| RTMK22.13 | pabaA1 biA1 pyroA4 metG1 ΔppoA::metG veA trpC801 | This study |
| RDIT58.21 | argB2 ΔppoA::trpC pyroA4 metG1 veA trpC801 | This study |
| RDIT55.7 | pyroA4 veA trpC801 | This study |
| TDIT11.12 | argB2 ΔppoC::trpC ppoC::pyroA methG1 veA trpC801 | This study |
| RDIT58.3 | ΔppoC::trpC pyroA4 veA trpC801 | This study |
| TU85 | pabaA1 biA1 argB2 pyroA4 ΔbrlA::argB veA | L. Yager |
| RDIT2.1 | metG1 veA | This study |
| RDIT86.7 | argB2 ΔbrlA::argB veA | This study |
| KHH52 | pabaA1 yA2 ΔargB::trpC ΔnsdD::argB trpC801 | 21 |
| KHH62 | pabaA1 yA2 ΔargB::trpC niiA(p)::nsdD trpC801 | 21 |
| Prototrophic isogenic strains | ||
| RDIT9.32 | veA | 55 |
| RDIT12.9 | metG1 ΔppoA::metG veA | 55 |
| RDIT58.12 | ΔppoC::trpC veA trpC801 | This study |
| RDIT54.7 | ΔppoC::trpC metG1 ΔppoA::metG veA trpC801 | This study |
| RDIT92.6 | ΔppoC::trpC ppoC::pyroA veA trpC801 | This study |
| RDIT81.10 | metG1 ΔppoA::metG veA gpdA(p)::ppoA::trpC | 55 |
| RRAW5.2 | argB2 ΔodeA::argB veA | R.A. Wilson |
| RDIT87.8 | niiA(p)::nsdD veA | This study |
| RDIT88.13 | ΔnsdD::argB veA | This study |
a
Strains starting with a “T” are original transformants, and strains beginning with an “R” are recombinants after a sexual cross. Some of the strains are not described in the text but were used for sexual crosses to create the final prototrophic strains.