FIG.2.

F. oxysporum FoSTUA gene. (A) Map of the FoSTUA locus. The bars pointing to the right indicate FoSTUA with introns (white segments). The 0.8-kb BglII-SalI fragment of FoSTUA was cloned in pBluescript KS(+) to make pS1BS, which was used as a probe for RNA gel blot analysis. Arrowheads above FoSTUA denote the orientations and locations of the oligonucleotide primers (Sf1 and Sr1) used in RT-PCR experiments. B, BglII; H, HindIII; S, SalI. (B) Similarity of FoStuA to other proteins. The amino acid sequence of FoStuA (Fo) was aligned with those of Asm-1 of N. crassa (Nc; Genbank accession no. CAD70882), StuA of A. nidulans (An; accession no. AAA33325), and StuA of P. marneffei (Pm; accession no. AAM27919). Amino acids that are conserved between FoStuA and any of the others are indicated as white letters on a black background. The most conserved bHLH motifs among the four sequences are underlined (amino acids 84 to 184 of FoStuA).