FIG. 6.
Western blot analysis of the expression of PMK1 and GFP-PMK1 constructs. Total protein was isolated from mycelia of the pmk1 deletion mutant (nn78), the wild-type strain (Guy11), or transformants of nn78 expressing GFP-PMK1 (Xh14), GFP-PMK1K53R (K53R-110), or GFP-PMK1AEF (AEF-4). When probed with the anti-Pmk1 antiserum (top panel), a 42-kDa Pmk1 band was observed in Guy11. In Xh14, AEF-4, and K53R-110, a larger protein of 68 kDa corresponding to the GFP-Pmk1 fusion was detected. When probed with an anti-GFP antibody (middle), a 68 kDa band was detected in Xh14, AEF-4, and K53R-110 but not in Guy11. The bottom panel was detection with an antiactin antibody to show that a similar amount of total protein was loaded in each lane.