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. 2004 Dec;3(6):1525–1532. doi: 10.1128/EC.3.6.1525-1532.2004

FIG. 7.

FIG. 7.

Overexpression of PMK1 mutant alleles resulted in morphological defects during appressorium formation. (A) Western blot analyses with total proteins extracted from the wild-type strain (Guy11) or transformants expressing the PMK1K53R (MK36-2) or GFP-PMK1AEF (MK37-3) fusion construct under the RP27 promoter. When probed with the anti-Pmk1 antiserum, MK36-2 and MK37-3 had two bands corresponding to the native Pmk1 and GFP-Pmk1 fusion proteins. A separate blot containing total protein from strain MT37-12 probed with anti-Pmk1 contains similar levels of protein between the native PMK1 and the GFP fusion. Blots probed with antiactin antibody demonstrated the relative variance in total proteins loaded in each lane. (B) Branching germ tubes formed by MK36-2 and MK37-3. Conidia from 10-day-old cultures were incubated at room temperature for 24 h and examined with differential intererence contrast (DIC) or epifluorescence microscopy. MT36-17 expressing GFP-PMK1K53R fusion under the native Pmk1 promoter had no morphological defects during appressorium formation.