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. 2017 Apr 6;8(4):1101–1111. doi: 10.1016/j.stemcr.2017.03.011

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This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Fluorescent Cell Barcoding Can Distinguish between High- and Low-Quality iPSCs

(A) One hESC line (HUES9), one high-quality iPSC line (NDC1), and one low-quality iPSC line (CV-hiPS-F) were barcoded, stained with TRA-1-81 and TRA-1-60 separately, and subsequently analyzed for GFP expression in three replicates (depicted as R1, R2, R3). Only data for staining with TRA-1-81 are shown.

(B and C) The HUES9-R3 hESC (B) displays no GFP⁺ cells and (C) has 97.7% of cells TRA-1-81⁺.

(D and E) The NDC1-R1 high-quality iPSC line (D) displays no GFP⁺ cells and (E) has 96.9% of cells TRA-1-81⁺.

(F and G) The CV-hiPS-F-R2 low-quality iPSC line has (F) GFP⁺ cells, indicating retrovirus reactivation and (G) a lower fraction of TRA-1-81⁺ cells (86.3%).

FSC-W, forward-scattered light width. See also Figure S1.