FIG. 4.

Telomere length was determined in different strains carrying the elg1Δ mutation. Chromosomal DNAs from each strain were digested with XhoI and hybridized with the telomeric repeat probe that can detect the Y′ class of telomeres. (A) Mutations in telomere maintenance genes had synergistic effects on telomere size when combined with the elg1Δ mutation. (B) S-phase checkpoint sensor gene mutations along with the elg1Δ mutation generated different telomere size changes. (C) The elg1Δ mutation in strains defective in S-phase checkpoint transducer genes also changed telomere length differently. (D) An additional mutation in the RAD24 gene in strains carrying elg1Δ and genes functioning in the DNA replication checkpoint slightly decreased telomere size.