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. 2004 Dec;3(6):1454–1463. doi: 10.1128/EC.3.6.1454-1463.2004

FIG. 4.

FIG. 4.

Real-time RT-PCR and Western blot analysis of gene and protein expression in a strain expressing activated CPG-1 (QL1) and the Δcprgs-1 strains ΔRGS-H8, -H15, and -H22. (A) Western blot analysis of CPG-1 protein accumulation. The arrow indicates the bands corresponding to CPG-1. The asterisk indicates a slower-migrating band that cross-reacts with α-CPG-1 antibody (40). Size markers are indicated in kilodaltons. (B) Western blot analysis of CPGB-1 protein accumulation. (C) Transcript accumulation of cprgs-1, cryparin (crp), cpg-1, and cpgb-1 in mutant strains relative to accumulation in the wild-type strain EP155. The arrowhead represents the reference value of 1.0 for EP155, calculated as described in Materials and Methods. Bars represent the standard error for each data set.