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. 2004 Dec;3(6):1454–1463. doi: 10.1128/EC.3.6.1454-1463.2004

FIG. 6.

FIG. 6.

Western blot and real-time RT-PCR analysis of protein and gene expression in EP155 and strains overexpressing cprgs-1. (A) S-tagged CPRGS-1 accumulation in RSO strains. Wild-type strain EP155 was loaded as negative control. Size markers are indicated in kilodaltons. (B) Western blot analysis of CPG-1 protein accumulation. The arrow indicates the bands corresponding to CPG-1. The asterisk indicates a slower-migrating band that cross-reacts with α-CPG-1 antibody (40). (C) Western blot analysis of CPGB-1 protein accumulation. (D) Transcript accumulation of cprgs-1, cryparin (crp), cpg-1, and cpgb-1 in mutant strains, relative to accumulation in wild-type strain EP155. The arrowhead represents the reference value of 1.0 for EP155. Bars represent standard error for each data set.