TABLE 1.
Oligonucleotides used in this studya
| Purpose and oligonucleotide | Sequence (5′-3′) |
|---|---|
| PCR-based cloning of cprgs-1 | |
| RGS-F2 | TGYGARYTNAAYATHGAYCA |
| RGS-R3 | TDRAAYTTNGGNACYGARTC |
| Mutated alleles of cpg-2 and cpg-3 | |
| C2-QLF | GTTGGAGGATTACGAAGCGAG |
| C2-QLR | CTCGCTTCGTAATCCTCCAAC |
| C3-QLF | GTCGGTGGGCTGCGATCAGAG |
| C3-QLR | CTCTGATCGCAGCCCACCGAC |
| C2-FH | TATAAGCTTCAAGATGGGTGCCTGC |
| C2-RS | TATGCATGCGTGATCACAAAATGCCTG |
| C3-FH | TATAAGCTTACAATGTGCTTCGGCAG |
| C3-RS | TATGCATGCTCAAACTCATAAGATCAG |
| S-tag constructs | |
| Stag-NF | TATATGCATAAAGAAACCGCTGCTGCT |
| Stag-NR | ATACTGCAGGCTGTCCATGTGCTGGCG |
| RGS-HF | ATAAAGCTTCACCTGCCGAAAATCG |
| RGS-NR | ATAGCGGCCGCGGTGAAAAGAGCGC |
| Real-time RT-PCR | |
| Cryp-probe | TCTACTTGCGCCACGTCCGGC |
| Cryp-F | CAGCGCATCAAGCTTCGA |
| Cryp-R | CGTGCAGCACTTGGCATCT |
| RGS-probe | ACGCCCAACGGCAAGGATCTCA |
| RGS-F | ACCGACCAAGCACTCCATTTA |
| RGS-R | CGCGAGTTCATGCCATTG |
a
Nucleotides in boldface introduce the desired mutation. Underlined nucleotides indicate restriction sites used for cloning.