FIG. 4.

Analysis of ergosterol in CaUPC2 deletion strains. Wild-type and UPC2 deletion strains were grown in YAD-CSM medium supplemented with 20 mg of uridine/ml, 50 mg of arginine/ml, and 20 mg of histidine/ml for 48 h, followed by a heptane extraction and spectrophotometric scan to detect ergosterol levels. Extracts were scanned from 230 to 310 nm, and the data were plotted. Strains are represented as BWP17 (open diamonds), UPC2 heterozygous deletion strain S-7 (open squares), UPC2 homozygous deletion strain D-6 (open triangles), and reconstructed strain EC-2 (open circles). The EC-7 strain had levels equivalent to S-7 and EC-2 but was omitted for clarity (data not shown). Abs., absorption.